ALLERGY-RELATED CHANGES IN LEVELS OF CD8(+)CD25(+)FOXP3(bright)TREG CELLS AND FOXP3 mRNA EXPRESSION IN PERIPHERAL BLOOD: THE ROLE OF IL-10 OR TGF-beta

作者：Eusebio M^*; Kuna P; Kraszula L; Kupczyk M; Pietruczuk M

来源：Journal of Biological Regulators and Homeostatic Agents, 2014, 28(3): 461-470.

• IL-10
• TGE-beta
• allergy
• asthma
• CD8(+) Treg cells

There is an increasing body of evidence that alterations of regulatory T (T-reg) cell numbers and functions lead to immune disorders. Accordingly, understanding the regulatory mechanisms that maintain peripheral regulatory T (T-reg) cell homeostasis is key to the development of effective targeted biologic therapies. We previously demonstrated the effects of IL-10 or TGF-beta on distinct CD8(+)CD28(-) T cell subsets in vitro. Allergy-related changes of CD8(+)CD25(+)FoxP3(bright)T(reg) cells and FoxP3 mRNA expression in peripheral blood were assessed by means of multicolor flow cytometry and real-time polymerase chain reaction (RT-PCR). Co-stimulation of CD8(+)CD25(+) T cells with anti-CD3/CD28 in the presence of either IL-10 or TGF-beta increased the frequency of CD8(+)CD25(+)FoxP3(bright) T-reg cells in patients with asthma and controls. Likewise, CD8(+)CD25(+) T cell activation with anti-CD3/CD28 and TGF-beta increased FoxP3mRNA expression in all groups. Anti-CD3/CD28 and IL-10 appeared to regulate FoxP3 mRNA expression in a phenotype-dependent manner. Specifically, co-stimulation by anti-CD3/CD28 and IL-10 markedly increased FoxP3 mRNA expression in the severe asthma group whereas it had opposite effects on this value in other groups. Taken altogether, these data suggest that IL-10 and TGF-beta may modulate FoxP3 expressions at the protein and mRNA levels in respect to their need for peripheral tolerance.

• 出版日期2014-9