Distribution and in Vitro Availability of Selenium in Selenium-Containing Storage Protein from Selenium-Enriched Rice Utilizing Optimized Extraction

作者：Fang, Yong; Catron, Brittany; Zhang, Yaofang; Zhao, Liyan; Caruso, Joseph A.; Hu, Qiuhui^*

来源：Journal of Agricultural and Food Chemistry, 2010, 58(17): 9731-9738.

DOI：10.1021/jf100934p

• Selenium
• rice protein
• distribution
• availability
• extraction

Selenium (Se) distribution in Se-enriched rice and optimization of extraction for Se-containing protein were studied. Se availability in Se-containing protein product was simulated using an in vitro gastrointestinal digestion. The results showed that Se was predominately found as organic Se, whereas inorganic Se comprised only 2.85% of the total Se. The glutelin fraction contained the largest amount of Se, approximately 31.3% of the total Se in the rice gain. Utilizing orthogonal analysis, the optimum extraction conditions were selected at a volume to weight of 20:1, 0.08 M NaOH, an extraction time of 3 h, and at a temperature of 35 C. A Se-containing rice protein product with 83.5% protein and 9.09 mu g g(-1) Se was sequestered using the optimal extraction method. This rice protein product with high molecular weight Se-containing protein can readily be digested to low molecular weight peptides and selenomethionine (52.3% of total Se in protein extract).

• 出版日期2010-9-8
• 单位南京财经大学; 南京农业大学