Multi-centre evaluation of real-time multiplex PCR for detection of carbapenemase genes OXA-48, VIM, IMP, NDM and KPC

作者：van der Zee Anneke; Roorda Lieuwe; Bosman Gerda; Fluit Ad C; Hermans Mirjam; Smits Paul H M; van der Zanden Adri G M; Witt Rene te; van Coppenraet Lesla E S Bruijnesteijn; Stuart James Cohen; Ossewaarde Jacobus M

来源：BMC Infectious Diseases, 2014, 14(1): 27.

DOI：10.1186/1471-2334-14-27

• Real-time multiplex PCR
• Carbapenemases
• OXA-48
• VIM
• IMP
• NDM
• KPC

Background: Resistance to carbapenem antibiotics is emerging worldwide among Enterobacteriaceae. To prevent hospital transmission due to unnoticed carriage of carbapenemase producing micro-organisms in newly admitted patients, or follow-up of patients in an outbreak setting, a molecular screening method was developed for detection of the most prevalent carbapenemase genes; bla(OXA-48), bla(VIM), bla(IMP), bla(NDM), and bla(KPC).
Methods: A real-time multiplex PCR assay was evaluated using a collection of 86 Gram negative isolates, including 62 carbapenemase producers. Seven different laboratories carried out this method and used the assay for detection of the carbapenemase genes on a selection of 20 isolates.
Results: Both sensitivity and specificity of the multiplex PCR assay was 100%, as established by results on the strain collection and the inter-laboratory comparisons.
Conclusions: In this study, we present a multiplex real-time PCR that is a robust, reliable and rapid method for the detection of the most prevalent carbapenemases bla(OXA-48), bla(VIM), bla(IMP), bla(NDM), and bla(KPC), and is suitable for screening of broth cultured rectal swabs and for identification of carbapenemase genes in cultures.

• 出版日期2014-1-14