Aptamer-based colorimetric detection of platelet-derived growth factor using unmodified gold nanoparticles

作者：Chang Chia Chen; Wei Shih Chung; Wu Tzu Heng; Lee Chung Han; Lin Chii Wann^*

来源：Biosensors and Bioelectronics, 2013, 42: 119-123.

DOI：10.1016/j.bios.2012.10.072

• Platelet-derived growth factor
• Aptamer
• Gold nanoparticle
• Colorimetry
• Base stacking
• Label-free

We developed a simple method for the detection of platelet-derived growth factors (PDGFs) based on base stacking effect coupled with an unmodified gold nanoparticle (AuNP) indicator. In the absence of a target, an aptamer probe and a capture probe stably co-exist in a solution, as it is difficult to sustain an interaction between both these probes due to the short 8 bp duplex. However, when a target protein binds to the aptamer probe, the strong base stacking effect can lead to a favorable and stable interaction between the aptamer and capture probes. Hence, the capture probe dissociates from the AuNP surfaces, inducing AuNP aggregation. Compared with other AuNP-based aptasensors for PDGFs, using this base stacking effect can overcome a structured-aptamer method's limitation of requiring thiolated-aptamer-modified, AuNPs. Under optimal detection conditions, this label-free colorimetric sensor could detect PDGFs down to 6 nM with high selectivity in the presence of other interferring proteins. This simple detection approach provides viable methods for a structured-aptamer sensing protocol.

• 出版日期2013-4-15