Novel synthesis of gold nanoclusters templated with L-tyrosine for selective analyzing tyrosinase

作者：Yang Xiaoming^*; Luo Yawen; Zhuo Yan; Feng Yuanjiao; Zhu Shanshan

来源：Analytica Chimica Acta, 2014, 840: 87-92.

DOI：10.1016/j.aca.2014.05.050

• Tyrosine
• Gold nanoclusters
• Tyrosinase
• Fluorescence quenching

L-Tyrosine (Tyr), playing roles as both a reducing reagent and a protecting ligand, has been first employed for synthesizing fluorescent gold nanoclusters (AuNCs@Tyr) via a novel one-pot strategy. The as-prepared AuNCs@Tyr exhibited a fluorescence emission at 470 nm with a quantum yield of approximately 2.5%. Subsequently, the AuNCs@Tyr described here was applied for detections of tyrosinase (TR) activity, which was based on the mechanism of aggregations of AuNCs@Tyr occurring on the active sites of TR since TR was introduced, thus leading to the fluorescence quenching of AuNCs@ Tyr. Accordingly, TR was analyzed in a linear range of 0.5-200 u mL(-1) with a detection limit of 0.08 u mL(-1) at a signal-to-noise ratio of 3. Significantly, TR has been considered as a critical marker for melanoma owing to its specifically expressing in melanoma cells. Therefore, this analytical method towards investigating TR activity may broaden avenues for meaningfully clinical applications.

• 出版日期2014-8-20
• 单位西南大学