Phosphatidylinositol transfer protein-alpha in platelets is inconsequential for thrombosis yet is utilized for tumor metastasis

作者：Zhao Liang; Thorsheim Chelsea L; Suzuki Aae; Stalker Timothy J; Min Sang H; Lian Lurong; Fairn Gregory D; Cockcroft Shamshad; Durham Amy; Krishnaswamy Sriram; Abrams Charles S^*

来源：Nature Communications, 2017, 8(1): 1216.

DOI：10.1038/s41467-017-01181-4

Platelets are increasingly recognized for their contributions to tumor metastasis. Here, we show that the phosphoinositide signaling modulated by phosphatidylinositol transfer protein type alpha (PITP alpha), a protein which shuttles phosphatidylinositol between organelles, is essential for platelet-mediated tumor metastasis. PITP alpha-deficient platelets have reduced intracellular pools of phosphoinositides and an 80% reduction in IP3 generation upon platelet activation. Unexpectedly, mice lacking platelet PITP alpha form thrombi normally at sites of intravascular injuries. However, following intravenous injection of tumor cells, mice lacking PITP alpha develop fewer lung metastases due to a reduction of fibrin formation surrounding the tumor cells, rendering the metastases susceptible to mucosal immunity. These findings demonstrate that platelet PITP alpha-mediated phosphoinositide signaling is inconsequential for in vivo hemostasis, yet is critical for in vivo dissemination. Moreover, this demonstrates that signaling pathways within platelets may be segregated into pathways that are essential for thrombosis formation and pathways that are important for non-hemostatic functions.

• 出版日期2017-10-31