NF kappa B and Survivin-Mediated Radio-Adaptive Response

作者：Grdina David J^*; Murley Jeffrey S; Miller Richard C; Woloschak Gayle E; Li Jian Jian

来源：Radiation Research, 2015, 183(4): 391-397.

DOI：10.1667/RR14002.1

A survivin-mediated radio-adaptive response was induced in SA-NH murine sarcoma cells following activation of nuclear transcription factor kappa B (NF kappa B) by very low doses of ionizing radiation of 5, 20 or 100 mGy. SA-NH cells and a clone stably transfected with a plasmid containing a mutated I kappa B alpha gene that prevents the activation of NF kappa B (SA-NH+mI kappa B alpha 1) were used to investigate the role of NF kappa B activation in the development and expression of the survivin-mediated radio-adaptive response. Tumor cells were exposed to very low doses of radiation 30 min prior to or at times ranging from 30 min to 6 h after the first of two 2 Gy doses separated by 24 h under in vitro conditions. Evidence of very low dose radiation induced a radio-adaptive response only in SA-NH but not SA-NH+mI kappa B alpha 1 cells was shown by both an increase in SA-NH cell survival of 20-40% using a standard colony forming assay and reduced apoptosis frequencies of 20-40% as determined by the TUNEL assay. Changes in survivin protein levels as a function of irradiation conditions were monitored by Western blot. A 100 mGy exposure 30 min prior to a 2 Gy dose resulted in an elevation in total survivin protein 24 h later in SA-NH but not SA-NH+mI kappa B alpha 1 cells. Transfection of cells with survivin siRNA inhibited elevation of survivin protein by very low dose radiation and the subsequent radio-adaptive response in SA-NH cells. These data suggest that the survivin-mediated radio-adaptive response is dependent upon the ability of cells to activate NF kappa B.

• 出版日期2015-4
• 单位西北大学