A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex

作者:Hewitt Sarah H; Parris Jonathan; Mailhot Romain; Butler Stephen J
来源:Chemical communications, 2017, 53(94): 12626-12629.
DOI:10.1039/c7cc05887j

摘要

A stable Eu(iii) complex is reported that binds reversibly to ATP and ADP, providing a ratiometric luminescent signal that enables the kinase-catalysed conversion of ATP to ADP to be monitored in real-time.

  • 出版日期2017-12-7