An immunomagnetic separation based fluorescence immunoassay for rapid myoglobin quantification in human blood

作者：Wang, Yi; Sun, Hongmei; Li, Rui; Ke, Peng; Zhu, Hongda; Guo, Huiling; Liu, Mingxing^*; Sun, Honghao

来源：Analytical Methods, 2016, 8(40): 7324-7330.

DOI：10.1039/c6ay01760f

• DOPED SILICA NANOPARTICLES
• ULTRASENSITIVE DETECTION
• GOLD NANOPARTICLES
• DIAGNOSTICS
• ANTIBODIES
• ANTIGEN
• WORLD
• ASSAY

Magnetic nanoparticle (NPs) and fluorescent NP pairs were designed for myoglobin (Myo) detection in human blood. Magnetic NPs MNP@SiO2@BSA@Au with about 200 nm diameter (by TEM) were prepared through a layer by layer technique following by adsorption of anti-Myo antibody 4E2 on a Au surface. The MNPs were well dispersed in an aqueous solution. Fluorescent NPs were prepared by conjugating fluorescent Rhodamine Red-X (RhX) and another Myo antibody 7C3 on silica NPs with a final diameter of 80 nm. In the presence of Myo, magnetic NPs bearing 4E2 and fluorescent NPs bearing 7C3 form a sandwich structure, which can be separated from blood under a magnetic field. The concentration of Myo is quantified by the fluorescence intensity of SNP@RhX NPs in the sandwich structure. The calibration curve of the Myo showed a linear range between 0 and 250 ng mL(-1) (R-2 = 0.993). The minimum detectable concentration was 0.28 ng mL(-1). The immunomagnetic separation based fluorescence immunoassay offered a simple and accurate way to determine Myo in human blood in 30 min.

• 出版日期2016
• 单位湖北工业大学