Anti-tumor effects of depleting and non-depleting anti-CD27 monoclonal antibodies in immune-competent mice

作者：Sakanishi Tamami; Yagita Hideo^*

来源：Biochemical and Biophysical Research Communications, 2010, 393(4): 829-835.

DOI：10.1016/j.bbrc.2010.02.092

• CD27
• T-cell co-stimulation
• Antibody therapy
• Cytotoxic T lymphocytes

CD27 plays an important role in T-cell co-stimulation and is also expressed on lymphomas. In the present study, we generated novel depleting and non-depleting monoclonal antibodies (mAbs) against mouse CD27 and characterized their co-stimulatory activity in vitro and anti-tumor effects in immune-competent mice bearing syngeneic T-cell lymphoma (EG7) expressing or lacking CD27. A profound anti-tumor effect was observed with a non-depleting mAb (RM27-3E5), but not with a depleting mAb (RM27-3C1), against either EG7/CD27(+) or EG7/CD27(-) tumors, which was associated with the induction of EG7-specific cytotoxic T lymphocytes (CTLs). Consistently, the anti-tumor effect of RM27-3E5 was abolished in T cell-deficient nude mice. These results indicate that a non-depleting agonistic mAb against CD27 is promising for cancer therapy by co-stimulating tumor-specific CTL induction.

• 出版日期2010-3-19