Development and validation for identity testing of I-DNADuo, a combination of I-DNA1 and a new multiplex system, I-DNA2

作者：Odriozola Adrian; Aznar Jose Maria; Celorrio David; Bravo Maria Luisa; Builes Juan Jose; Martinez de Pancorbo Marian

来源：International Journal of Legal Medicine, 2012, 126(1): 167-172.

DOI：10.1007/s00414-011-0585-2

• Forensic science
• Mini and midi short tandem repeat
• STR
• DNA typing
• Multiplex
• CODIS
• Combination

The I-DNADuo multiplex system combination is composed of previously validated I-DNA1 and a new short tandem repeat (STR) multiplex named I-DNA2 that analyses 11 STR loci plus amelogenin. I-DNADuo, with amplicon sizes ranging from 57 to 298 bp, is specifically designed to analyse amelogenin and 15 STR loci (ten of them plus amelogenin in duplicate), including all the STR loci of the CODIS, ISSL and ECL databases, and seven of the eight in GCL. The validation of I-DNADuo shows that it is a highly sensitive, robust multiplex system for obtaining individual genetic profiles and for detecting and preventing allelic dropouts.

• 出版日期2012-1