Gonadal status-dependent effects of in vivo beta-estradiol administration to female rats on in vitro epileptiform activity induced by low [Mg2+](o) in combined hippocampus-entorhinal cortex slices

作者：Veliskova Jana^*; Velisek Libor

来源：Epilepsy Research, 2013, 107(3): 297-301.

DOI：10.1016/j.eplepsyres.2013.09.002

• Epileptiform discharges
• Neuronal excitability
• beta-Estradiol
• Female rats
• Dentate gyrus network
• In vitro electrophysiology

There are controversial data regarding estrogen effects on neuronal excitability. We investigated whether beta-estradiol (EB) administration to ovariectomized (OVX) or gonadally intact female rats alters epileptiform activity within the dentate gyrus network induced in vitro by removing [Mg2+](o) in combined hippocampus-entorhinal cortex slices. In vivo EB administration significantly influenced the epileptifornn activity in gonadal status-dependent manner. The onset of epileptiform discharges was modestly delayed in slices from OVX rats replaced with physiologically relevant doses of EB but the number of discharges was not affected. In contrast, EB administration to gonadally intact rats had robust effects such that: EB delayed the onset of discharges but significantly increased their number within the dentate gyrus network. Our data suggest that EB in physiologically relevant concentrations does not seem to negatively affect hippocampal neuronal excitability, nevertheless supraphysiological EB levels may enhance seizure severity.

• 出版日期2013-12