A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection

作者:Schwessinger Benjamin; Li Xiang; Ellinghaus Thomas L; Chan Leanne Jade G; Wei Tong; Joe Anna; Thomas Nicholas; Pruitt Rory; Adams Paul D; Chern Maw Sheng; Petzold Christopher J; Liu Chang C*; Ronald Pamela C*
来源:Integrative Biology, 2016, 8(4): 542-545.
DOI:10.1039/c5ib00232j

摘要

Posttranslational modification (PTM) of proteins and peptides is important for diverse biological processes in plants and animals. The paucity of heterologous expression systems for PTMs and the technical challenges associated with chemical synthesis of these modified proteins has limited detailed molecular characterization and therapeutic applications. Here we describe an optimized system for expression of tyrosine-sulfated proteins in Escherichia coli and its application in a bio-based crop protection strategy in rice.

  • 出版日期2016