Protective Effect of Daidzin against D-Galactosamine and Lipopolysaccharide-Induced Hepatic Failure in Mice

作者：Kim Sung Hwa; Heo Jeong Haing; Kim Yeong Shik; Kang Sam Sik; Choi Jae Sue; Lee Sun Mee^*

来源：Phytotherapy Research, 2009, 23(5): 701-706.

DOI：10.1002/ptr.2710

• apoptosis
• daidzin
• D-galactosamine
• LPS
• liver failure
• oxidative stress
• TNF-alpha

This study examined the effects of daidzin, a major isoflavone from Puerariae Radix, on D-galactosamine (D-GalN) and lipopolysaccharide (LPS)-induced liver failure. Mice were given an intraperitoneal injection of daidzin (25, 50, 100 and 200 mg/kg) 1 h before receiving an injection of D-GalN (700 mg/kg)/LPS (10 mu g/kg). Daidzin markedly reduced the elevated serum aminotransferase activity and the levels of lipid peroxidation and tumor necrosis factor-alpha. The glutathione content was lower in the D-GalN/LPS group, which was attenuated by daidzin. The daidzin pretreatment attenuated the swollen mitochondria observed in the D-GalN/LPS group. Daidzin attenuated the apoptosis of hepatocytes, which was confirmed using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling method and a caspase-3 assay. Overall, these results suggest that the liver protection of daidzin is due to reduced oxidative stress and its antiapoptotic activity.

• 出版日期2009-5