A system for omega-transaminase mediated (R)-amination using L-alanine as an amine donor

作者：Richter N; Farnberger J E; Pressnitz D; Lechner H; Zepeck F; Kroutil W

来源：Green Chemistry, 2015, 17(5): 2952-2958.

DOI：10.1039/c4gc02363c

Chiral amines are important building blocks for fine chemicals and pharmaceuticals. Consequently, various biocatalytic routes in particular using omega-transaminases (omega-TAs) have been developed recently. Although catalysts for the synthesis of both enantiomers are available, the application of alanine dependent (R)-selective omega-TAs is less favourable due to the requirement of the more expensive D-alanine as an amine donor. Here we describe an efficient method for (R)-amination using omega-TAs in combination with an alanine racemase (AlaR). In this case, the readily available L-alanine can be used as an amine donor leading to improved atom efficiency and significantly reduced costs.

• 出版日期2015