A propagation procedure for Cuphea aequipetala Cav. (Lythraceae) and antioxidant properties of wild and greenhouse-grown plants

作者：Martinez Bonfil Blanca P; Pineda Montero Mariela; Lopez Laredo Alma R; Salcedo Morales Guadalupe; Evangelista Lozano Silvia; Trejo Tapia Gabriela^*

来源：Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas, 2013, 12(1): 1-14.

• antioxidant activity
• Cuphea aequipetala
• flavonoids
• Lythraceae
• phenolic compounds
• shoot proliferation
• callus

Cuphea aequipetala Cav. (Lythraceae) is native to Mexico and is used in folk medicine to treat tumors. An efficient protocol for in vitro shoot proliferation and plant acclimatization of C. aequipetala was developed. Total phenolic compounds and flavonoids contents were determined in methanolic extracts of roots, stems, and leaves from wild and greenhouse-grown plants. Their antioxidant properties were compared using in vitro assays (scavenging of 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals, and reducing power in the phosphomolybdenum assay). This is the first report of a successful propagation procedure for C. aequipetala. These methods offer a viable approach for long-term in vitro conservation and proliferation of this species. C. aequipetala shoots maintained their proliferation capacity during longterm subculture (3 years). The propagated shoots can successfully acclimatize and grow to maturity, and they retain the ability to accumulate antioxidants.

• 出版日期2013-1