A new approach for the in vitro identification of the cytotoxicity of superparamagnetic iron oxide nanoparticles

作者：Mahmoudi Morteza^*; Simchi Abdolreza; Imani Mohammad; Shokrgozard Mohammad A; Milani Abbas S; Haefeli Urs O; Stroeve Pieter

来源：Colloids and Surfaces B: Biointerfaces , 2010, 75(1): 300-309.

DOI：10.1016/j.colsurfb.2009.08.044

• Iron oxide nanoparticles
• SPION
• Toxicity
• DMEM
• MTT

Superparamagnetic iron oxide nanoparticles (SPIONs) aie increasingly used in medical applications, such as targeting delivery and imaging In the future, patients are more likely to be exposed to pharmaceutical products containing such particles The study of toxicity of SPIONs has become of great importance in recent years, although the published data in this arena is limited. The aim of the present work is to investigate the cytotoxicity of SPIONs and the effect of the particles on t he cell medium components For this purpose, uncoated and polyvinyl alcohol (PVA) coated SPIONs with narrow size distribution were synthesized via a well-known coprecipitation method. The mouse fibroblast cell line L929 was exposed to SPIONs to probe the toxicity of magnetic nanoparncles during the bio application Changes to the cell medium caused by SPIONs were analyzed with zeta potential measurements, ultraviolet visible spectroscopy (UV/vis) and the 3-[4,5-chmethylthiazol-2y11-2,5-chphenyltetrazolium bromide (MU) assay It is observed that gas vesicles are formed in SPION-treated cells Toxicity is conventionally explained by changes in the DMEM's pH and composition due to the tendency of SPIONs to interact with biomolecules A new procedure is proposed to examine

• 出版日期2010-1-1