A low-molecular-weight galactofucan from the seaweed, Spatoglossum schroederi, binds fibronectin and inhibits capillary-like tube formation in vitro

作者：Menezes Maira Maria; Duarte Barreto Nobre Leonardo Thiago; Rossi Gustavo Rodrigues; Almeida Lima Jailma; Melo Silveira Raniere Fagundes; Cavichiolo Franco Celia Regina; Trindade Edvaldo Silva; Nader Helena Bonciani; Oliveira Rocha Hugo Alexandre

来源：International Journal of Biological Macromolecules, 2018, 111: 1067-1075.

DOI：10.1016/j.ijbiomac.2018.01.119

• Fucoidan
• Brown seaweed, endothelial cells
• Anti-angiogenic

A low-molecular-weight (LMW) heterofucan (designated fucan B) was obtained from the brown seaweed, Spatoglossum schroederi, and its activity as an inhibitor of capillary-like tube formation by endothelial cells (ECs) was analyzed. Chemical, infrared and electrophoretic analyses confirmed the identity of fucan B. In contrast to other LMW fucans, fucan B (0.012-0.1 mg/mL) inhibited ECs capillary-like tube formation in a concentration dependent manner. In addition, fucan B (0.01-0.05 mg/mL) did not affect ECs proliferation. Fucan B also inhibited ECs migration on a fibronectin-coated surface, but not on laminin- or collagen-coated surfaces. Biotinylated fucan B was used as a probe to identify its localization. Confocal microscopy experiments revealed that biotinylated fucan did not bind to the cell surface, but rather only to fibronectin. Our findings suggest that fucan B inhibits ECs capillary-like tube formation and migration by binding directly to fibronectin and blocking fibronectin sites recognized by cell surface ligands. However, further studies are needed to evaluate the in vivo effects of fucan B.

• 出版日期2018-5