A chicken liver cell line efficiently supports the replication of ALV-J possibly through its high level viral receptor and efficient protein expression system

作者：Li, Tuofan; Xie, Jing; Lv, Lu; Sun, Shu; Dong, Xiaomei; Xie, Quan; Liang, Guangcheng; Xia, Chichao; Shao, Hongxia; Qin, Aijian^*; Ye, Jianqiang^*

来源：Veterinary Research, 2018, 49(1): 41.

DOI：10.1186/s13567-018-0537-7

• AVIAN-LEUKOSIS VIRUS
• SUBGROUP-J
• CHINA
• ANTIGEN
• P27

In this study, we identified a chicken liver cell line (LMH) which could strongly support the replication of ALV-J (Subgroup J of avian leukosis virus) with high viral titer. Notably, ALV-J was efficiently detected by ELISA in LMH cells 1 day before DF1 cells. In comparison with DF1 cells, LMH cells not only expressed higher levels of ALV-J receptor chNHE-1, but also possessed a more efficient protein expression system for foreign genes. Thus, LMH cells could be a novel tool to shorten the ALV-J eradication approach and accelerate studies on the pathogenesis and oncogenesis of ALV-J.

• 出版日期2018-5-2
• 单位扬州大学