An EST-SSR marker linked with yellow rust resistance in wheat

作者：Ercan S; Ertugrul F; Aydin Y; Akfirat F S; Hasancebi S; Akan K; Mert Z; Bolat N; Yorgancilar O; Altinkut Uncuoglu A^*

来源：Biologia Plantarum, 2010, 54(4): 691-696.

DOI：10.1007/s10535-010-0122-z

• breeding
• Puccinia striiformis f. sp tritici
• Triticum aestivum

Expressed sequenced tags containing simple sequence repeats (EST-SSRs) were used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.). A cross between yellow rust resistant (PI178383) and susceptible (Harmankaya99) wheat genotypes was performed and respective DNA pools from the resistant and susceptible F(2) seedlings were constructed. 78 EST-SSR primers were used for bulked segregant analysis and one EST-SSR marker (Pk54), identified as 200 bp fragment, was present in the resistant parent and resistant F(2) hybrids but not in the susceptible ones. 108 wheat genotypes differing in yellow rust resistance were screened with Pk54 and 68 % of the wheat genotypes, known to be yellow rust resistant, had the Pk54 marker, further suggesting that the presence of this marker correlates with yellow rust resistance.

• 出版日期2010-12