A study on a chitosan-gelatin-hyaluronic acid scaffold as artificial skin in vitro and its tissue engineering applications

作者：Liu HF; Mao JS; Yao KD^*; Yang GH; Cui L; Cao YL

来源：Journal of Biomaterials Science, Polymer Edition, 2004, 15(1): 25-40.

DOI：10.1163/156856204322752219

• tosan-gelatin-hyaluronic acid scaffold
• keratinocyte
• fibroblasts
• co-culture
• artificial skin

Chitosan-gelatin-hyaluronic acid scaffolds for tissue regeneration were fabricated by freezing and lyophilizing methods. The scaffolds showed a higher water uptake and retention abilities than chitosan-gelatin scaffolds did. Fibroblasts cultured in chitosan-gelatin-hyaluronic acid scaffolds grew and proliferated well, and they exhibited a strong viability. Keratinocytes were co-cultured with fibroblasts in chitosan-gelatin-hyaluronic acid scaffolds to construct an artificial bilayer skin in vitro. The artificial skin obtained was flexible and had good mechanical properties. The data from this study suggested that chitosan-gelatin-hyaluronic acid scaffolds are suitable for preparing a bilayer skin substitute.

• 出版日期2004
• 单位天津大学; 上海组织工程研究与开发中心