alpha V-Integrins Are Required for Mechanotransduction in MDCK Epithelial Cells

作者：Teravainen Terhi P; Myllymaki Satu M; Friedrichs Jens; Strohmeyer Nico; Moyano Jose V; Wu Chuanyue; Matlin Karl S; Muller Daniel J; Manninen Aki^*

来源：PLos One, 2013, 8(8): e71485.

DOI：10.1371/journal.pone.0071485

The properties of epithelial cells within tissues are regulated by their immediate microenvironment, which consists of neighboring cells and the extracellular matrix (ECM). Integrin heterodimers orchestrate dynamic assembly and disassembly of cell-ECM connections and thereby convey biochemical and mechanical information from the ECM into cells. However, the specific contributions and functional hierarchy between different integrin heterodimers in the regulation of focal adhesion dynamics in epithelial cells are incompletely understood. Here, we have studied the functions of RGD-binding alpha V-integrins in a Madin Darby Canine Kidney (MDCK) cell model and found that alpha V-integrins regulate the maturation of focal adhesions (FAs) and cell spreading. alpha V-integrin-deficient MDCK cells bound collagen I (Col I) substrate via alpha 2 beta 1-integrins but failed to efficiently recruit FA components such as talin, focal adhesion kinase (FAK), vinculin and integrin-linked kinase (ILK). The apparent inability to mature alpha 2 beta 1-integrin-mediated FAs and link them to cellular actin cytoskeleton led to disrupted mechanotransduction in alpha V-integrin deficient cells seeded onto Col I substrate.

• 出版日期2013-8-19