Augmented Macrophage Differentiation and Polarization of Tumor-associated Macrophages Towards M1 Subtype in Listeria-administered Tumor-bearing Host

作者：Rai Rakesh K; Vishvakarma Naveen K; Mohapatra Tribhuban M; Singh Sukh Mahendra^*

来源：Journal of Immunotherapy, 2012, 35(7): 544-554.

DOI：10.1097/CJI.0b013e3182661afa

• Listeria
• myelopoiesis
• tumor-associated macrophages
• tumor microenvironment
• tumor progression

This study investigates the effect of Listeria administration on differentiation of macrophages from precursor bone marrow cells and functional status of tumor-associated macrophages (TAM). Listeria administration not only resulted in an augmented infiltration of tumor by F4/80 (+) macrophages but also repolarized the functional status of TAM displaying features of some M1 macrophage subtype with upregulated phagocytosis and tumoricidal activity accompanied by altered expression of monocarboxylate transporter-1, toll-like receptor-2, surface markers: CD11c, interleukin-2 receptor, CD62L, and secreted molecules: nitric oxide, interleukin (IL)-1, IL-6, tumor necrosis factor-alpha, and vascular endothelial growth factor. Declined tumor cell survival and modulated repertoire of cytokines: interferon-gamma, IL-6, IL-10, and transforming growth factor-beta in tumor microenvironment indicated their role in polarization of TAM towards proinflammatory state. Bone marrow cell of Listeria-administered tumor-bearing mice showed augmented survival, declined expression of p53 upregulated modulator of apoptosis with an upregulated differentiation into activation responsive bone marrow-derived macrophages along with altered expression of macrophage-colony stimulating factor, macrophage-colony stimulating factor receptor, and granulocyte macrophage-colony stimulating factor receptor. These findings indicate that Listeria infection is associated with an augmented differentiation of macrophages accompanied by tumoricidal activation of TAM.

• 出版日期2012-9
• 单位河北医科大学