Anti-proliferation and migration efficacy via inhibition of an androgen synthesis key enzyme in castration-resistant prostate cancer

作者：Zhu Sha; Li Sanqiang; Song Dongkui

来源：Journal of Chemical and Pharmaceutical Research, 2014, 6(3): 372-377.

• CRPC
• Diagnostic biomarkers
• Down-regulation
• DU145
• Gene differential expressions
• Prostate cancer cells
• UGT2B15-shRNA
• Wound healing assays

After evaluating by gene differential expression microarray we found that UGT2B15is highly expressed in castration resistant prostate cancer cell lines. We supposed that docetaxel in combination with UGT2B15downregulation may have a synergistic effect on tumor cells proliferation and migration. This study aimed to investigate the effects of UGT2B15silencingon the sensitivity of prostate cancer cells to docetaxel treatment. After transfected with UGT2B15-targeted shRNAs and treated with different concentrations of docetaxel, proliferation and migration of DU145 cells were examined by enumeration in a haemocytometerand wound healing assay. Expression of UGT2B15 protein in DU145cells was detected by western blot. Results demonstrated that silencing of UGT2B15 promoted docetaxel induced cell growth inhibition and inhibited cell migration in CRPC. Results indicate that UGT2B15may be used as a new promising diagnostic biomarker and a potential anticancer therapeutic target for CRPC.

• 出版日期2014
• 单位河南大学; 郑州大学