A statistical test for conserved RNA structure shows lack of evidence for structure in lncRNAs

作者：Rivas, Elena^*; Clements, Jody; Eddy, Sean R.

来源：Nature Methods, 2017, 14(1): 45-48.

DOI：10.1038/NMETH.4066

• LONG NONCODING RNAS
• SECONDARY STRUCTURE
• PREDICTION
• INFORMATION
• PHYLOGENY

Many functional RNAs have an evolutionarily conserved secondary structure. Conservation of RNA base pairing induces pairwise covariations in sequence alignments. We developed a computational method, R-scape (RNA Structural Covariation Above Phylogenetic Expectation), that quantitatively tests whether covariation analysis supports the presence of a conserved RNA secondary structure. R-scape analysis finds no statistically significant support for proposed secondary structures of the long noncoding RNAs HOTAIR, SRA, and Xist.

• 出版日期2017-1