Asiaticoside hinders the invasive growth of keloid fibroblasts through inhibition of the GDF-9/MAPK/Smad pathway

作者：Wu, Xin; Bian, Difei; Dou, Yannong; Gong, Zhunan; Tan, Qian; Xia, Yufeng; Dai, Yue^*

来源：Journal of Biochemical and Molecular Toxicology, 2017, 31(8): e21922.

DOI：10.1002/jbt.21922

• asiaticoside
• GDF-9
• keloid
• TGF-1

Higher expression of growth differentiation factor-9 (GDF-9) in keloids compared with hypertrophic scars and normal skin tissues has been reported recently. The present study was performed to investigate the role of GDF-9 in keloid pathogenesis, and to elucidate its implication for asiaticoside in the keloid management. The data showed that GDF-9 could enhance the proliferation, migration, and invasion of keloid fibroblasts (KFs), while it only slightly elevated collagen expression, indicating that the effect of GDF-9 was opposite to that of TGF-1. The bioactivity difference between GDF-9 and TGF-1 could be explained by the different phosphorylated sites on the downstream Smad2/3. Moreover, asiaticoside could inhibit GDF-9-induced activation of MAPKs and Smad pathway in KFs. In conclusion, GDF-9 enhanced the invasive growth of KFs, which was achieved by phosphorylation of Smad 2/3 at the linker region through activation of MAPKs pathway. Asiaticoside hindered the invasive growth of KFs by inhibiting the GDF-9/MAPK/Smad pathway.

• 出版日期2017-8
• 单位中国药科大学; 南京大学; 南京师范大学