Amination of Ketones by Employing Two New (S)-Selective omega-Transaminases and the His-Tagged omega-TA from Vibrio fluvialis

作者：Mutti Francesco G; Fuchs Christine S; Pressnitz Desiree; Turrini Nikolaus G; Sattler Johann H; Lerchner Alexandra; Skerra Arne; Kroutil Wolfgang^*

来源：European Journal of Organic Chemistry, 2012, 2012(5): 1003-1007.

DOI：10.1002/ejoc.201101476

• Amines
• Amination
• Ketones
• Transaminases
• Biocatalysis

Two recently identified (S)-selective ?-transaminases (?-TAs) that originate from Paracoccus denitrificans (Strep-PD-?TA, cloned with an N-terminal Strep-tag II) and Pseudomonas fluorescens (PF-?TA) were employed for the asymmetric amination of selected prochiral ketones. The substrates tested were transformed into optically pure amines (%26gt;99?% ee) with high conversion (up to %26gt;99?%). The ?-TAs led to higher conversion in the absence of dimethyl sulfoxide as a cosolvent than in its presence (15?%, v/v). Additionally, it was shown that a His-tagged recombinant transaminase from Vibrio fluvialis (His-VF-?TA, cloned with an N-terminal His6-tag) showed for a single substrate, ethyl acetoacetate, significantly higher stereoselectivity for the amination compared to the corresponding commercial enzyme preparation (%26gt;99 vs. 50?%).

• 出版日期2012-2