The Macrophage Is a Key Factor in Renal Injuries Caused by Glomerular Hyperfiltration

作者：Sasaki Motofumi; Shikata Kenichi; Okada Shinichi^*; Miyamoto Satoshi; Nishishita Shingo; Kataoka Hitomi Usui; Sato Chikage; Wada Jun; Ogawa Daisuke; Makino Hirofumi

来源：Acta Medica Okayama, 2011, 65(2): 81-89.

• kidney
• inflammation
• chemokine

Glomerular hyperfiltration is a common pathway leading to glomerulosclerosis in various kinds of kidney diseases. The 5/6 renal ablation is an established experimental animal model for glomerular hyperfiltration. On the other hand, low-grade inflammation is also a common mechanism for the progression of kidney diseases including diabetic nephropathy and atherosclerosis. Here we analyzed the gene expression profile in the remnant kidney tissues of 5/6 nephrectomized mice using a DNA microarmy system and compared it with that of sham-operated control mice. The 5/6 nephrectomized mice showed glomerular hypertrophy and an increase in the extracellular matrix in the glomeruli. DNA microarray analysis indicated the up-regulated expression of various kinds of genes related to the inflammatory process in remnant kidneys. We confirmed the up-regulated expression of platelet factor-4, and monocyte chemoattractant protein-1, 2, and 5 in remnant kidneys by RT-PCR. The current results; suggest that the inflammatory process is involved in the progression of glomerulosclerosis and is a common pathway of the pathogenesis of kidney disease.

• 出版日期2011-4