Aspirin-triggered lipoxin A4 attenuates lipopolysaccharide induced inflammatory response in primary astrocytes

作者：Yao, Chengye; Yang, Dong; Wan, Zhenzhen; Wang, Zhenxing; Liu, Rengang; Wu, Yan; Yao, Shanglong; Yuan, Shiying; Shang, You^*

来源：International Immunopharmacology, 2014, 18(1): 85-89.

DOI：10.1016/j.intimp.2013.10.028

• Astrocyte
• Lipopolysaccharide
• Aspirin-triggered-lipoxinA(4)
• Neuroinflammation

The activation of astrocytes contributes to inflammatory responses underlying brain injury and neurodegenerative diseases. Lipoxins have emerged as mediators of endogenous anti-inflammatory events. However, the involvement of aspirin-triggered-lipoxin A(4) (ATL) in astrocyte-induced neuroinflammatory responses has not been investigated. Here, we examined the anti-inflammatory effects of ATL in the central nervous system using rat astrocyte cultures stimulated with lipopolysaccharide (LPS). We found that pretreatment with ATL exerted potent anti-inflammatory effects by inhibiting LPS-induced production of nitric oxide and prostaglandin E-2. ATL also reduced the expression of cyclooxygenase 2 and inducible nitric oxide synthase mRNA and protein. Furthermore, ATL suppressed the LPS-induced translocation of the NF-kappa B p65 subunit to the nucleus and prevented LPS-induced I kappa B alpha phosphorylation in a dose-dependent manner. These findings suggest that ATL attenuates neuroinflammation by inhibiting the NF-kappa B signal transducer pathway in cultured cortical astrocytes.

• 出版日期2014-1
• 单位华中科技大学