Direct electrochemistry of glucose oxidase immobilized on TiO2-graphene/nickel oxide nanocomposite film and its application

作者：Xu, Chun-Xuan; Huang, Ke-Jing^*; Chen, Xue-Min; Xiong, Xiao-Qin

来源：Journal of Solid State Electrochemistry, 2012, 16(12): 3747-3752.

DOI：10.1007/s10008-012-1813-5

• TiO2-graphene
• Nickel oxide nanoparticles
• Glucose
• Glucose oxidase
• Biosensor

A novel electrochemical platform based on nickel oxide (NiO) nanoparticles and TiO2-graphene (TiO2-Gr) was developed for the direct electrochemistry of glucose oxidase (GOD). The electrochemical behavior of the sensor was studied using cyclic voltammetry and chronoamperometry. The experimental results demonstrated that the nanocomposite well retained the activity of GOD and the modified electrode GOD/NiO/TiO2-Gr/GCE exhibited excellent electrocatalytic activity toward the redox of GOD as evidenced by the significant enhancement of redox peak currents in comparison with bare GCE. The biosensor responded linearly to glucose in the range of 1.0-12.0 mM, with a sensitivity of 4.129 mu A mM(-1) and a detection limit of 1.2 x 10(-6) M under optimized conditions. The response time of the biosensor was 3 s. In addition, the developed biosensor possessed good reproducibility and stability, and there was negligible interference from other electroactive components.

• 出版日期2012-12
• 单位信阳师范学院