Microdosimetry for conventional and supra-electroporation in cells with organelles

作者：Gowrishankar TR; Esser AT; Vasilkoski Z; Smith KC; Weaver JC^*

来源：Biochemical and Biophysical Research Communications, 2006, 341(4): 1266-1276.

DOI：10.1016/j.bbrc.2006.01.094

• nanosecond scale pulses
• megavolt-per-meter fields
• supra-electroporation
• conventional electroporation
• organelles
• cell system model
• microdosimetry

Conventional electroporation (EP) by 0.1 to 1 kV/cm pulses longer than 100 mu s, and supra-electroporation by 10 to 300 kV/cm pulses shorter than I lis cause different cellular effects. Conventional EP delivers DNA, proteins, small drugs, and fluorescent indicators across the plasma membrane (PM) and causes moderate levels of phosphatidylserine (PS) translocation at the PM. We hypothesize that supra-EP is central to intracellular effects such as apoptosis induction and higher levels of PS translocation. Our cell system model has 20,000 interconnected local models for small areas of the PM and organelle membranes, small regions of aqueous media, appropriate resting potentials, and the asymptotic EP model. Conventional EP primarily affects the PM, but with a hint of endoplasmic reticulum involvement. Supra-EP can involve all of a cell's membrane at the largest fields. Conventional EP fields tend to go around cells, but supra-EP fields go through cells, extensively penetrating organelles.

• 出版日期2006-3-24