Direct Electrochemistry of Hemoglobin and its Electrocatalysis Based on a Carbon Nanotube Paste Electrode

作者：Zhai Zi Qin; Wu Jie; Sun Wei^*; Jiao Kui

来源：Journal of the Chinese Chemical Society, 2009, 56(3): 561-567.

DOI：10.1002/jccs.200900083

• Hemoglobin
• Carbon nanotubes
• Carbon paste electrode
• Direct electrochemistry
• Electrocatalysis

A new hemoglobin (Hb) and carbon nanotube (CNT) modified carbon paste electrode was fabricated by simply mixing the Hb, CNT with carbon powder and liquid paraffin homogeneously. To prevent the leakage of Hb from the electrode surface, a Nafion film was further applied on the surface of the Hb-CNT composite paste electrode. The modified electrode was characterized by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). Direct electrochemistry of hemoglobin in this paste electrode was easily achieved and a pair of well-defined quasi-reversible redox peaks of a heme Fe(III)/Fe(II) couple appeared with a formal potential (E(0)) of -0.441 V (vs. SCE) in pH 7.0 phosphate buffer solution (PBS). The electrochemical behaviors of Hb in the composite electrode were carefully studied. The fabricated modified bioelectrode showed good electrocatalytic ability for reduction of H(2)O(2) and trichloroacetic acid (TCA), which shows potential applications in third generation biosensors.

• 出版日期2009-6
• 单位青岛科技大学