Multilevel Differential Control of Hormone Gene Expression Programs by hnRNP L and LL in Pituitary Cells

作者：Lei Lei; Cao Wenguang; Liu Ling; Das Urmi; Wu Yujia; Liu Guodong; Sohail Muhammad; Chen Yangjun; Xie Jiuyong

来源：Molecular and Cellular Biology, 2018, 38(12): UNSP e00651.

DOI：10.1128/MCB.00651-17

• hnRNP paralogs
• divergence
• prolactin
• growth hormone
• RNA-Seq
• cryptic splicing
• intron
• alternative splicing
• hnRNP

The pituitary-derived somatolactotrophe GH(3) cells secrete both growth hormone (GH) and prolactin (PRL). We have found that the hnRNP L and L-like (LL) paralogs differentially regulate alternative splicing of genes in these cells. Here, we show that hnRNP L is essential for PRL only, but LL is essential for both PRL and GH production. Transcriptome-wide RNA sequencing (RNA-Seq) analysis indicates that they differentially control groups of hormone or hormone-related genes involved in hormone production/regulation at total transcript and alternative exon levels. Interestingly, hnRNP L also specifically binds and prevents the aberrant usage of a non-conserved CA-rich intron piece of Prl pre-mRNA transcripts, and many others involved in endocrine functions, to prevent mostly cryptic last exons and mRNA truncation. Essential for the full hnRNP L effect on specific exons is a proline-rich region that emerged during evolution in vertebrate hnRNP L only but not LL. Together, our data demonstrate that the hnRNP L and its paralog, LL, differentially control hormone gene expression programs at multiple levels, and hnRNP L in particular is critical for protecting the transcriptome from aberrant usage of intronic sequences. The multilevel differential control by hnRNPs likely tailors the transcriptome to help refine and safeguard the different gene expression programs for different hormones.

• 出版日期2018-6