Determination of Cephradine Capsules Content Based on Fluorescence Quenching Method of FOS-μSIA-LOV

作者：Qiu Xin; Li Xin-xia; Iburaim, Arkin; Kuerban, Gulina; Zhang Li-hua; Li Yan^*

来源：Spectroscopy and Spectral Analysis, 2017, 37(11): 3475-3478.

DOI：10.3964/j.issn.1000-0593(2017)11-3475-04

• FOS
• mu STA
• Cefradine
• Fluorescence

The content of cephradine capsules was determined by a system of FOS-pSIA-LOV(fiber optic sensor-micro sequential injection-valve laboratory), based on the principle of cefradine quenching fluorescein in methanol solvent, optimize experimental conditions. The order of inhalation was anhydrous methanol, cefradine, fluorescein and anhydrous methanol; the carrier liquid suction volume was 500 mu L; cefradine inhalation volume was 100 mu L. Cefradine quenched fluorescein fluorescence intensity and concentration in 0. 05 similar to 0.5 mg.mL(-1) linear relationship as Delta F=812. 6+284. 41, r=0. 999 5, precision RSD was 4. 4%, the recovery was 101%similar to 103%. FOS-SIA-LOV and personalized drug testing and programming resulted in automated on-line process analysis with the good reproducibility, less reagent consumption, time and labor saving.

• 出版日期2017-11
• 单位新疆医科大学