Non-invasive cell type selective in vivo monitoring of insulin resistance dynamics

作者：Paschen Meike; Moede Tilo; Leibiger Barbara; Jacob Stefan; Bryzgalova Galyna; Leibiger Ingo B^*; Berggren Per Olof^*

来源：Scientific Reports, 2016, 6(1): 21448.

DOI：10.1038/srep21448

Insulin resistance contributes to the development of cardio-vascular disease and diabetes. An important but unresolved task is to study the dynamics of insulin resistance in selective cell types of insulin target tissues in vivo. Here we present a novel technique to monitor insulin resistance dynamics non-invasively and longitudinally in vivo in a cell type-specific manner, exemplified by the pancreatic beta-cell situated within the micro-organ the islet of Langerhans. We utilize the anterior chamber of the eye (ACE) as a transplantation site and the cornea as a natural body-window to study the development and reversibility of insulin resistance. Engrafted islets in the ACE that express a FoxO1-GFP-based biosensor in their beta-cells, report on insulin resistance measured by fluorescence microscopy at single-cell resolution in the living mouse. This technique allows monitoring of cell type specific insulin sensitivity/resistance in real-time in the context of whole body insulin resistance during progression and intervention of disease.

• 出版日期2016-2-22