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摘要
Background: Adipose-derived stromal cells hold promise for skeletal tissue engineering. However, various studies have observed that adipose-derived stromal cells differ significantly in their biology depending on species of derivation. In the following study, the authors sought to determine the species-specific response of adipose-derived stromal cells to recombinant TGF-beta 1 (rTGF-beta 1).
Methods: Adipose-derived stromal cells were derived from mouse and human sources. Recombinant TGF-beta 1 was added to culture medium (2.5 to 10 ng/ml); proliferation and osteogenic and adipogenic differentiation were assessed by standardized parameters, including cell counting, alkaline phosphatase, alizarin red, oil red O staining, and quantitative real-time polymerase chain reaction.
Results: Recombinant TGF-beta 1 was found to significantly repress cellular proliferation in both mouse and human adipose-derived stromal cells (p < 0 01). Recombinant TGF-beta 1 was found to significantly repress osteogenic differentiation in mouse adipose-derived stromal cells. In contrast, osteogenic differentiation of human adipose-derived stromal cells proceeded unimpeded in either the presence or the absence of rTGF-beta 1. Interestingly, rTGF-beta 1 induced expression of a number of osteogenic genes in human adipose-derived stromal cells, Including BMP2 and BMP4
Conclusions: The authors' results further detail an important facet in which mouse and human adipose-derived stromal cells differ. Mouse adipose-derived stromal cell osteogenesis is completely inhibited by rTGF-beta 1, whereas human adipose-derived stromal cell osteogenesis progresses in the presence of rTGF-beta 1. These data highlight the importance of species of derivation in basic adipose-derived stromal cell biology. Future studies will examine in more detail the species-specific differences among adipose-derived stromal cell populations. (Plast. Reconstr Surg. 126 412, 2010.)
- 出版日期2010-8
