The Implication of Substance P in the Development of Tendinopathy: A Case Control Study

作者：Han Soo Hong; Choi Wonchul; Song Jiye; Kim Jaehee; Lee Seungyong; Choi Youngrak; Byun Seong Eun; Ahn Taekeun; Ahn Heejung; Ding Catherine; Baik Lloyd; Ward Spencer; Ting Kang; Lee Soonchul

来源：International Journal of Molecular Sciences, 2017, 18(6): 1241.

DOI：10.3390/ijms18061241

• tendinopathy
• pathogenesis
• substance P
• type 3 collagen

It was reported that substance P had beneficial effects in the healing of acute tendon injury. However, the relationship between substance P and degenerative tendinopathy development remains unclear. The purpose of this study was to determine the role of substance P in the pathogenesis of tendinopathy. Healthy and tendinopathy tendon were harvested from human and tenocytes were cultured individually. The expression levels of genes associated with tendinopathy were compared. Next, substance P was exogenously administered to the healthy tenocyte and the effect was evaluated. The results showed that tendinopathy tenocytes had higher levels of COL3A1, MMP1, COX2, SCX, ACTA2, and substance P gene expression compared to healthy tenocytes. Next, substance P treatment on the healthy tenocyte displayed similar changes to that of the tendinopathy tenocytes. These differences between the two groups were also determined by Western blot. Additionally, cells with substance P had the tendinopathy change morphologically although cellular proliferation was significantly higher compared to that of the control group. In conclusion, substance P enhanced cellular proliferation, but concomitantly increased immature collagen ( type 3 collagen). Substance P plays a crucial role in tendinopathy development and could be a future therapeutic target for treatment.

• 出版日期2017-6