Direct electron transfer from glucose oxidase immobilized on a nano-porous glassy carbon electrode

作者：Haghighi Behzad^*; Tabrizi Mahmoud Amouzadeh

来源：Electrochimica Acta, 2011, 56(27): 10101-10106.

DOI：10.1016/j.electacta.2011.08.106

• Nano-porous glassy carbon electrode
• Activated glassy carbon electrode
• Direct electron transfer
• Glucose oxidase
• Glucose biosensor

A pair of well-defined and reversible redox peaks was observed for the direct electron transfer (DET) reaction of an immobilized glucose oxidase (GOx) on the surface of a nano-porous glassy carbon electrode at the formal potential (E degrees') of -0.439 V versus Ag/AgCl/saturated KCl. The electron transfer rate constant (k(s)) was calculated to be 5.27 s(-1). The dependence of E degrees' on pH indicated that the direct electron transfer of the GOx was a two-electron transfer process, coupled with two-proton transfer. The results clearly demonstrate that the nano-porous glassy carbon electrode is a cost-effective and ready-to-use scaffold for the fabrication of a glucose biosensor.

• 出版日期2011-11-30