Activity-driven mobilization of post-synaptic proteins

作者：Gutierrez R Carolina; Flynn Robyn; Hung Johanna; Kertesz Audrey C; Sullivan Andrea; Zamponi Gerald W; El Husseini Alaa; Colicos Michael A^*

来源：European Journal of Neuroscience, 2009, 30(11): 2042-2052.

DOI：10.1111/j.1460-9568.2009.07007.x

• actin cytoskeleton
• neuroligin
• neuronal plasticity
• PSD-95
• rat
• Shank

Synapses established during central nervous system development can be modified through synapse elimination and formation. These processes are, in part, activity dependent and require regulated trafficking of post-synaptic components. Here, we investigate the activity-driven remodeling of cultured rat hippocampal neurons at 14 days in vitro, focusing on the post-synaptic proteins PSD-95, Shank, neuroligin (NL)1 and actin. Using live imaging and photoconductive stimulation, we found that high-frequency activity altered the trajectory, but not velocity, of PSD-95-GFP and Shank-YFP clusters, whereas it reduced the speed and increased the number of NL1 clusters. Actin-CFP reorganized into puncta following activity and similar to 50% of new puncta colocalized with NL1 clusters. Actin reorganization was enhanced by the overexpression of NL1 and decreased by the expression of an NL1 mutant, NL1-R473C. These results demonstrate activity-dependent changes that may result in the formation of new post-synaptic sites and suggest that NL1 modulates actin reorganization. The results also suggest that a common mechanism underlies both the developmental and activity-dependent remodeling of excitatory synapses.

• 出版日期2009-12