Au-colloids-polypyrrole nanocomposite film based xanthine biosensor

作者：Devi Rooma; Yadav Sandeep; Pundir C S^*

来源：Colloids and Surfaces A: Physicochemical and Engineering Aspects , 2012, 394: 38-45.

DOI：10.1016/j.colsurfa.2011.11.021

• Xanthine
• Xanthine oxidase
• xanthine biosensor
• AuPPy nanocomposite
• Meat

Stable colloids of Au/polypyrrole (AuPPy) nanocomposites were prepared by oxidizing pyrrole monomer with HAuCl4 in a cetyltrimethylammonium bromide (CTAB) solution and cast on the platinum (Pt) electrode. Xanthine oxidase (XOD) was immobilized onto this nanocomposite film through glutaraldehyde. The AuPPy/Pt electrode was characterized by Fourier transform infrared (FTIR), cyclic voltammetry (CV), X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and electrochemical impedance spectroscopy (EIS) before and after immobilization of XOD. The XOD/AuPPy/Pt as working electrode, Ag/AgCl as reference electrode and Pt wire as auxiliary electrode were connected through a potentiostat to construct a xanthine biosensor. The biosensor exhibited optimum response within 4 s at pH 7.2 and 30 degrees C and linearity from 0.4 to 100 mu M for xanthine with a detection limit 0.4 mu M (S/N = 3). The fabricated biosensor was successfully employed for determination of xanthine in fish, chicken, pork, and beef meat. The biosensor showed only 40% loss of its initial activity after its 200 uses over 100 days, when stored at 4 degrees C.

• 出版日期2012-1-20