Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens

作者：Sittova Martina^*; Roederova Magdalena; Dendis Milos; Hricova Kristyna; Pudova Vendula; Horvath Radek; Ruzicka Filip; Dosoudilova Sarka; Kolar Milan

来源：Microbial Drug Resistance, 2015, 21(3): 352-357.

DOI：10.1089/mdr.2014.0210

The infections caused by extended-spectrum -lactamase (ESBL)-producing organisms are associated with increased mortality. The real-time polymerase chain reaction (PCR) method, which enables detection of ESBLs directly from patients' clinical material, was developed. This study focused on bla(CTX-M) and bla(SHV) determination in endotracheal aspirates. Each sample was identified with standard microbiological procedures and simultaneously analyzed for the presence of nucleic acids, which encode CTX-M and SHV ESBL enzymes using real-time PCR. A total of 341 samples were investigated. In the set, 27 ESBL-positive samples were identified by phenotypic methods, while 60 positive samples were identified by the PCR method. Of the 60 PCR-positive samples, 58 were positive for the bla(CTX-M). In two samples, the ESBL bla(SHV-ESBL) gene was detected. One phenotypically positive sample was PCR negative. The real-time PCR assay does not require a cultivation step and therefore enables detection of ESBL in 6 hours. The rapid method is necessary for early and adequate antimicrobial treatment.

• 出版日期2015-6-1