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摘要
This paper describes a sequential injection chromatography procedure for determination of picloram in waters exploring the low backpressure of a 2.5 cm long monolithic C(18) column. Separation of the analyte from the matrix was achieved in less than 60 s using a mobile phase composed by 20:80 (v v(-1)) acetonitrile: 5.0 mmol L(-1) H(3)PO(4) and flow rate of 30 mu L s(-1). Detection was made at 223 nm with a 40 mm optical path length cell. The limits of detection and quantification were 33 and 137 mu g L(-1), respectively. The proposed method is sensitive enough to monitor the maximum concentration level for picloram in drinking water (500 mu g L(-1)). The sampling frequency is 60 analyses per hour, consuming only 300 mu L of acetonitrile per analysis. The proposed methodology was applied to spiked river water samples and no statistically significant differences were observed in comparison to a conventional HPLC-UV method.
- 出版日期2010
