Does insulin impact cold-induced fluid- and protein-extravasation?

作者：Brekke Hege K^*; Hammer**org Stig; Nedrebo Torbjorn; Hu**y Paul

来源：Cryobiology, 2015, 70(2): 136-142.

DOI：10.1016/j.cryobiol.2014.12.009

• Hypothermia
• Inflammation
• Endothelial barrier function
• Fluid shifts
• Insulin

Background: Insulin has been shown to stabilize the endothelial barrier via inactivation of the endothelial contractile machinery and enhancement of cell-cell adhesions. Here we explored if insulin by its endothelial-stabilizing and anti-inflammatory properties could influence the increase of fluid- and protein-extravasation during hypothermia.
Methods: Two groups of animals (n = 10, each) were cooled to 28 degrees C, with insulin-infusion (I-group) or without (C-group), in a randomly controlled study. Fluid balance, hemodynamics, plasma volume (PV), colloid osmotic pressures in plasma (COPp) and interstitial fluid (COPi), hematocrit (Hct), cytokine profiles, serum-albumin- and protein-concentrations were measured and fluid extravasation rate (FER) and albumin-and protein-masses calculated.
Results: During 240 min of hypothermia the albumin-and protein-masses together with COPp decreased significantly in both groups. COPi remained essentially unchanged. Plasma volume decreased significantly in the C-group, whereas only a decreasing trend was present in the I-group. Hemoconcentration was significant in both study groups reflected by the Hct-values. A slight increasing trend of FER was seen in both groups from 0.10 (0.04) ml/kg/min and 0.09 (0.05) mg/kg/min, C-group and I-group, respectively, to 0.14 (0.05) mg/kg/min and 0.12 (0.03) mg/kg/min, during the hypothermic period. Between-group differences were absent for all listed parameters including FER.
Conclusion: Insulin administration does not impact fluid and protein extravasation significantly in animals undergoing cooling and prolonged hypothermia.

• 出版日期2015-4