Analysis of cantharidin in false blister beetles (Coleoptera: Oedemeridae) by headspace solid-phase microextraction and gas chromatography-mass spectrometry

作者：Mehdinia Ali^*; Asiabi Mina; Jabbari Ali; Abtahi S Mohammad

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2011, 879(27): 2897-2901.

DOI：10.1016/j.jchromb.2011.08.020

• Solid-phase microextraction
• Cantharidin
• False blister beetle
• Oedemeridae

A headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography-mass spectrometry (CC-MS) method was developed to determine a type of terpenoid named as cantharidin in the false blister beetles, family Oedemeridae. The experimental parameters for HS-SPME method were optimized. Six commercial fibers for HS-SPME method development were tested and the divinylbenzene/carboxene/polydimethylsiloxane fiber was selected to provide the best detection of analyzed compound. The calibration curve showed linearity in the range of 0.1-50 mu g mL(-1), correlation coefficient (R(2) = 0.992), limit of detection (0.01 ng mL(-1)) and quantitation (0.04 ng mL(-1)) were obtained for the proposed method. The relative standard deviations of intra-day and inter-day assays were 7.8 and 3.4%, respectively. The recovery values, obtained after spiking the beetle samples by three concentration levels of standard solution, were higher than 87%. The results indicated the successful application of the proposed method on the analysis of cantharidin from the false blister beetles.

• 出版日期2011-10-1