Mapping seedling resistance to net form of net blotch (Pyrenophora teres f. teres) in barley using detached leaf assay

作者：Koenig Janine; Perovic Dragan; Kopahnke Doris; Ordon Frank

来源：Plant Breeding, 2014, 133(3): 356-365.

DOI：10.1111/pbr.12147

• Pyrenophora teres f
• teres
• detached leaf test
• net blotch
• QTL
• DArT
• barley
• major gene
• single conidial lines

To develop molecular markers against Pyrenophora teres f. teres in barley, a detached leaf assay was conducted on two DH populations with a set of 11 single conidial lines (SCLs). Out of these, three showed different reactions in the DH population UschixHHOR3073%26apos; and two in (PxV)xHHOR9484%26apos;. For SCL QLB%26apos;, a 1r:1s (2=2.78) segregation was observed in the population UschixHHOR3073%26apos;. In contrast to this, a continuous variation was observed for the SCL WvB%26apos; and d8_4%26apos; in the DH population UschixHHOR3073%26apos; and for AR%26apos; and net1840%26apos; in (PxV)xHHOR9484%26apos;. With respect to resistance to the SCL QLB%26apos;, a single major gene was located on chromosome 7H, and for resistance against SCL WvB%26apos;, two QTLs were detected on chromosome 3H and 7H, and against SCL d8_4%26apos;, two loci were mapped on chromosome 3HS in UschixHHOR3073%26apos;. In the DH population (PxV)xHHOR9484%26apos;, one locus conferring resistance to the SCL AR%26apos; was located on chromosome 3H. For resistance to SCL net1840%26apos;, two QTLs were mapped on chromosome 4H and 5H.

• 出版日期2014-6