Antioxidant Activity of Ge-132, a Synthetic Organic Germanium, on Cultured Mammalian Cells

作者：Wada Takeyoshi; Hanyu Takashi; Nozaki Kota; Kataoka Kosuke; Kawatani Tomoro; Asahi Toru; Sawamura Naoya^*

来源：Biological & Pharmaceutical Bulletin, 2018, 41(5): 749-753.

DOI：10.1248/bpb.b17-00949

• organic germanium
• Ge-132
• hydrogen peroxide
• oxidative stress

Ge-132 is a synthetic organic germanium that is used as a dietary supplement. The antioxidant activity of Ge-132 on cultured mammalian cells was investigated in this study. First, Ge-132 cytotoxicity on mammalian cultured cells was determined by measuring lactate dehydrogenase (LDH) levels. Ge-132 had no cytotoxic effect on three different cell lines. Second, the cell proliferative effect of Ge-132 was determined by measuring ATP content of whole cells and counting them. Ge-132 treatment of Chinese hamster ovary (CHO-K1) and SH-SY5Y cells promoted cell proliferation in a dose-dependent manner. Finally, antioxidant activity of Ge-132 against hydrogen peroxide-induced oxidative stress was determined by measuring the levels of intracellular reactive oxygen species (ROS) and carbonylated proteins. Pre-incubation of CHO-Kl and SH-SY5Y cells with Ge-132 suppressed intracellular ROS production and carbonylated protein levels induced by hydrogen peroxide. Our results suggest that Ge-132 has antioxidant activity against hydrogen peroxide-induced oxidative stress.

• 出版日期2018-5