An optimized B lymphocyte stimulator (BLyS) antagonist peptide inhibits the interaction of BLyS with BCMA

作者：Tian Yu; Zhu Yan feng; Wu Zhen; Feng Jian nan; Li Yan; Shen Bei fen; Sun Jian^*

来源：Biotechnology Letters, 2013, 35(4): 523-528.

DOI：10.1007/s10529-012-1117-y

• Autoimmune diseases
• B cell maturation antigen (BCMA)
• B lymphocyte stimulator (BLyS)
• Computer-aided homology modeling

B lymphocyte stimulator (BLyS) antagonists are new therapeutic reagents for treating the autoimmune diseases. Peptibodies can inhibit the bioactivity of BLyS, the same as other BLyS antagonists: decoyed BLyS receptors and anti-BLyS antibodies. In this study, a new optimized BLyS antagonist peptide was designed according to our previous work by the computer-aided homology modeling. Competitive ELISA showed that the peptide at 100 mu g/ml could inhibit 54 % of the BCMA-Fc binding to BLyS. To maintain its stability and spatial conformation, the peptide was fused to human IgG1 Fc to form a peptide-Fc fusion protein-a novel peptibody by gene engineering. ELISA indicated that the peptibody could bind with BLyS in dosage-dependent manner as BCMA-Fc did. This study highlights the possibility of designing and optimizing BLyS antagonist peptides with high biopotency by the computer-aided design. Thus, these peptides could neutralize BLyS activity and be potential antagonists to treat autoimmune diseases related with BLyS overexpression.

• 出版日期2013-4
• 单位天津大学