科研之友
微信
新浪微博
Facebook
分享链接
摘要
To examine stabilizing effects of the base pair interaction on a protein scaffold, various peptides with L-alpha-amino acids bearing a nucleobase in the side chain (nucleobase ammo acids, NBAs) were designed based on a G-peptide beta-hairpin structure, and their conformational properties were investigated by circular dichroism and NMR spectroscopy Thermodynamic analyses based on the chemical shifts showed that adenine-thymine pairing in a diagonal fashion at positions 4 and 15 (2AT) enhanced thermal stability of the peptide conformation by more than 30 K as compared with the wild-type G-peptide In NOESY spectrum, not only numerous nonadjacent crosspeaks but also long-range crosspeaks between the nucleobases were observed in some peptides with the base pairing NMR structure calculations of the 2AT peptide confirmed that cross-strand pairing of the nucleobases occurs on the well-defined beta-hairpin structure as designed Taken together, the base
- 出版日期2010
