Detection of amplified SRY gene by a novel electrochemical biosensor based on gold nanoparticles

作者：Mazloum Ardakani M^*; Heidari M M; Naderi M; Sheikh Mohseni M A

来源：Scientia Iranica, 2012, 19(3): 913-918.

DOI：10.1016/j.scient.2012.01.009

• DNA hybridization
• Sex determining region of the Y gene (SRY)
• Au nanoparticles
• Voltammetry
• Biosensor

This paper describes an electrochemical hybridization biosensor that investigates DNA hybridization on the modified Glassy Carbon Electrode (GCE). GCE was modified by a mixture of Au nanoparticles (AuNPs) and calf thymus DNA (ctDNA). SH-ssDNA was self-assembled on a modified electrode, which is a segment of the sex determining region of the Y (SRY) gene. This electrode can detect the complementary and non-complementary strand of SRY gene, by Methylene Blue (MB), as an electroactive label, and the Differential Pulse Voltammetry (DPV) technique. The modified GCE for the complementary DNA had a linear dynamic range of 0.5-30.0 ppm. This is the first report of sex determination by the electrochemical biosensor of a Polymerase Chain Reaction (PCR) product. Also, this biosensor showed stability on thermal denaturation for five sequential denaturation processes.

• 出版日期2012-6