Adhesion mechanisms of Vibrio fluvialis to skin mucus of Epinephelus awoara

作者：Yan Qingpi; Zhao Minhui; Wang Xiaolu; Zou Wenzheng; Chen Changsheng^*

来源：Chinese Journal of Oceanology and Limnology, 2010, 28(2): 260-266.

DOI：10.1007/s00343-010-9229-5

• Vibrio fluvialis
• Epinephelus awoara
• skin mucus
• adhesion

Vibrio fluvialis incubated in trypticase soy broth (TSB) showed stronger adhesion to the skin mucus of Epinephelus awoara than V. fluvialis grown on trypticase soy agar (TSA), and this bacterial adhesion was assessed in terms of saturation kinetics. Treating bacteria with antibody against O-antigens resulted in significantly reduced bacterial adhesion. In the early growth stage, the adhering bacteria numbers increased with incubation time, peaked at 24 h, and then dropped sharply. Prior heat treatment of the mucus at various temperatures resulted in different effects on bacterial adhesion. No remarkable effect on bacterial adhesion was detected after treating mucus with protease K and trypsin, whereas periodic acid treatment significantly enhanced bacterial adhesion. Extracts of V. fluvialis surface structures at high concentrations significantly inhibited bacterial adhesion, while relatively low concentrations enhanced adhesion. V. fluvialis had strong affinity for a high molecular weight mucus component. These results indicate that V. fluvialis adhered strongly to E. awoara mucus by specific adhesion and that this adhesion was influenced by many factors, including bacterial conditions, mucus, and the environment.

• 出版日期2010-3
• 单位集美大学